Regeneration of plants from leaf explants of micropropagated clonal Eucalyptus grandis

Summary Callus production along with caulogenesis was obtained from leaf explants of micropropagated clonal Eucalyptus grandis after six to twelve weeks of culture. Out of eight clones tested, six were amenable to shoot production using simple media containing naphthaleneacetic acid and either 6-benzyladenine or zeatin. Differences in growth regulator requirements for organogenesis were observed between different clones. These shoots were then elongated on a medium containing gibberellic acid and rooted using media derived from the micropropagation medium. Light conditions were also found to be important for regeneration. This protocol is the first published on regeneration from nonseedling material and it will facilitate the Agrobacterium tumefaciens -mediated transformation of selected clonal Eucalyptus grandis.Abbreviations BA 6-benzyladenine - IBA indole-3-butyric acid - MS Murashige and Skoog (1962) medium - NAA naphthaleneacetic acid - PAR photosynthetically active radiation - PVP polyvinyl pyrrolidone - SH Schenk and Hildebrandt (1972) medium