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High activity xylanase production by Streptomyces olivaceoviridis E-86
Authors:C.H. Ding  Z.Q. Jiang  X.T. Li  L.T. Li  I. Kusakabe
Affiliation:(1) College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 294, Qinghua Donglu, 17, Haidian District, Beijing, 100083, China
Abstract:The effects of different factors on xylanase production by Streptomyces olivaceoviridis E-86 were studied under shake flask conditions. The best initial pH value of growth medium for xylanase production was pH 6.0. Corn cob xylan and beef peptone were the best C source and N source, respectively. The enzyme activity was doubled by addition of 1.5% (v/v) Tween-80 in the medium. By the combination of the above variables, the highest xylanase activity obtained was 1653 U/ml which is the highest ever reported from Streptomyces sp.
Keywords:Nitrogen source  pH  production  Streptomyces olivaceoviridis  Tween 80  xylan  xylanase
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