<Emphasis Type="Italic">Enterococcus faecium</Emphasis> isolated from honey synthesized bacteriocin-like substances active against different <Emphasis Type="Italic">Listeria monocytogenes</Emphasis> strains |
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Authors: | Carolina Ibarguren Raúl R Raya María C Apella M Carina Audisio |
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Institution: | (1) Department of Chemistry and Biotechnology, Agricultural University of Norway, P.O. Box 5040, N-1432 Ås, Norway; (Author for correspondence);(2) The Norwegian Food Research Institute, MATFORSK, Osloveien 1, N-1432 Ås, Norway |
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Abstract: | Four Enterococcus faecium strains, isolated from honeycombs (C1 and M2d strains) and feral combs (Mori1 and M1b strains) secreted antimicrobial substances
active against fourteen different Listeria spp. strains. The antimicrobial compound(s) present in the cell free supernatant were highly thermostable (121°C for 15 min)
and inactivated by proteolytic enzymes, but not by α-amylase and lipase, thus suggesting a peptidic nature. Since the structural
bacteriocin gene determinants of enterocins A and B were PCR amplified from the four E. faecium isolates, only the bacteriocin produced by strain C1 was further characterized: it showed a broad band of approximately 4.0–7.0
kDa in SDS-PAGE and was bactericidal (4 log decrease) against L. monocytogenes 99/287. L. monocytogenes 99/287R, a clone spontaneously resistant to the enterocin produced by E. avium DSMZ17511 (ex PA1), was not inhibited by the enterocin-like compounds produced by strain C1. However, it was inhibited in
mixed culture fermentations by E. faecium C1 and a bacteriostatic effect was observed. The bacteriocin-producer Enterococcus strains were not haemolytic; gelatinase negative and sensitive to vancomycin and other clinically relevant antibiotics. |
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