Brain Soluble Fractions Which Modulate Na+, K+-ATPase Activity Likewise Modify Muscarinic Receptor |
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Authors: | de Lores Arnaiz Georgina Rodríguez Schneider Patricia Peña Clara |
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Institution: | (1) Instituto de Biología Celular y Neurociencias Prof. Eduardo De Robertis , Facultad de Medicina, PROBICENE-CONICET, Universidad de Buenos Aires, Paraguay 2155, 1121- Buenos Aires, Argentina;(2) IQUIFIB, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113- Buenos Aires, Argentina |
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Abstract: | Two brain soluble fractions, named peaks I and II, which respectively stimulate and inhibit neuronal Na+, K+-ATPase activity, have been isolated by gel filtration in Sephadex G-50. Since cholinergic transmission seems related to such enzyme activity, in this study we evaluated the effect of brain peak I, peak II, a more purified fraction II-E and commercial ouabain, on specific binding of the muscarinic antagonist 3H]quinuclidinyl benzilate to membranes from rat cerebellum, hippocampus and cerebral cortex. We found that binding was increased by peak I and decreased by peak II, II-E and ouabain, all effects proving concentration-dependent. Since the changes exerted on the muscarinic receptor followed a pattern similar to the one already described for synaptosomal membrane Na+, K+-ATPase activity, both systems seem to interact at a functional level. |
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Keywords: | Muscarinic receptors endogenous modulators ouabain ATPase modulators CNS membranes [3H]QNB binding |
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