Comparison of excretory/secretory and circulating antigens of Toxoplasma gondii by enzyme immunoassay and immunoblotting. |
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Authors: | J Hafid H Raberin B Pozzetto Z Y Akono R Tran Manh Sung M Jana and O Gaudin |
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Institution: | Laboratoire de Parasitologie, Hôpital Nord, Saint-Etienne, 42277, Saint-Priest-en-Jarez, France |
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Abstract: |
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1992. Comparison of excretory/secretory and circulating antigens of Toxoplasma gondii by enzyme immunoassay and immunoblotting. International Journal for Parasitology 22: 1083–1088. Toxoplasma gondii trophozoites (RH strain) were cultured in embryonic fibroblasts in order to study the kinetics of production of excretory/secretory antigens, and the results were compared to the production of circulating antigens in an in vivo mouse model. By capture-ELISA, excretory/secretory antigens were first detected on the fourth day of culture whereas circulating antigens were first detected 1 day after infection. Similar concentrations of antigens were detected in both models as evidenced by comparable absorbance values. By immunoblotting, the excretory/secretory antigens were also detected later compared to circulating antigens (day 4 vs day 1). Seven major polypeptides were detected in both antigen preparations, six of them having the same molecular mass (110, 75,48, 30, 24 and 22 kDa). |
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Keywords: | Toxoplasma gondii excretory/secretory antigens circulating antigens capture-ELISA immunoblotting cell culture |
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