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Comparison of excretory/secretory and circulating antigens of Toxoplasma gondii by enzyme immunoassay and immunoblotting.
Authors:J Hafid  H Raberin  B Pozzetto  Z Y Akono  R Tran Manh Sung  M Jana and O Gaudin
Institution:

Laboratoire de Parasitologie, Hôpital Nord, Saint-Etienne, 42277, Saint-Priest-en-Jarez, France

Abstract:Image , Image , Image , Image , Image , Image and Image 1992. Comparison of excretory/secretory and circulating antigens of Toxoplasma gondii by enzyme immunoassay and immunoblotting. International Journal for Parasitology 22: 1083–1088. Toxoplasma gondii trophozoites (RH strain) were cultured in embryonic fibroblasts in order to study the kinetics of production of excretory/secretory antigens, and the results were compared to the production of circulating antigens in an in vivo mouse model. By capture-ELISA, excretory/secretory antigens were first detected on the fourth day of culture whereas circulating antigens were first detected 1 day after infection. Similar concentrations of antigens were detected in both models as evidenced by comparable absorbance values. By immunoblotting, the excretory/secretory antigens were also detected later compared to circulating antigens (day 4 vs day 1). Seven major polypeptides were detected in both antigen preparations, six of them having the same molecular mass (110, 75,48, 30, 24 and 22 kDa).
Keywords:Toxoplasma gondii  excretory/secretory antigens  circulating antigens  capture-ELISA  immunoblotting  cell culture
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