Effects of Estradiol and IGF-1 on the Sodium Calcium Exchanger in Rat Cultured Cortical Neurons |
| |
Authors: | Julio C Sánchez Diego F López-Zapata Liliana Francis Lina De Los Reyes |
| |
Institution: | 1.Grupo de Fisiología Celular y Aplicada, Facultad Ciencias de la Salud,Universidad Tecnológica de Pereira,Pereira,Colombia;2.Grupo de Modelos Experimentales para las Ciencias Zoohumanas, Facultad de Ciencias,Universidad del Tolima,Ibagué,Colombia |
| |
Abstract: | The Na+/Ca2+ exchanger (NCX) is an important bidirectional transporter of calcium in neurons and has been shown to be involved in neuroprotection.
Calcium can activate a number of cascades that can result in apoptosis and cell death, and NCX is a key factor in regulating
the cytoplasmic concentration of this ion. 17-β-estradiol and insulin-like growth factor 1 (IGF-1) are known neuroprotective
hormones with interacting mechanisms and effects on intracellular calcium; however, their relationship with the NCX has not
been explored. In this article, the effects of these two hormones on neuronal NCX were tested using the whole-cell patch clamp
technique on rat primary culture neurons. Both 17-β-estradiol and IGF-1 produced an increase in the NCX-mediated inward current
and a decrease in the NCX-mediated outward current. However, the IGF-1 effect was lower than that of 17-β-estradiol, and the
effect of both agents together was greater than the sum of each agent alone. Neither of the agents affected the pattern of
regulation by extracellular or intrapipette calcium. Inhibitors of the IGF-1 and 17-β-estradiol receptors and inhibitors of
the main signaling pathways failed to change the observed effects, indicating that these actions were not mediated by the
classical receptors of these hormones. These effects on the NCX could be a mechanism explaining the neuroprotective actions
of 17-β-estradiol and IGF-1, and these findings could help researchers to understand the role of the NCX in neuroprotection. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|