Aeration volume and photosynthetic photon flux affect cell growth and secondary metabolite contents in bioreactor cultures ofMorinda citrifolia |
| |
Authors: | Saifullah Ahmed Eun-Joo Hahn Kee-Yoeup Paek |
| |
Institution: | (1) Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763 Cheongju, Korea |
| |
Abstract: | To improve their growth and secondary metabolite production, we culturedMorinda citrifolia leaf cells for 3 weeks in bioreactors with different aeration volumes (0.05, 0.1, 0.2, or 0.3 vvm; or 0.05/0.1/0.2/0.3 vvm,
as increased at 5-d interval), and photosynthetic photon fluxes (PPF; 0, 15, 30, or 45 μ,moL m-2 s-1). Cell growth was greatest (15.6 g L-1 dry weight) at 0.3 vvm whereas the accumulation of secondary metabolites (total anthraquinones, phenolics, and flavonoids)
was maximized at 0.1 vvm. A PPF of 15 μmoLm-2 s-1 accelerated the accumulation of both cell biomass and metabolites. Dark-culturing suppressed cell growth, while a high PPF
(45 μmoLm-2 s-1) inhibited metabolite biosynthesis. Further studies are required to understand the reason for differences in the effect of
light on cell growth and secondary metabolite contents inM. citrifolia cell cultures. |
| |
Keywords: | aeration volume anthraquinones cell culture flavonoids Morinda citrifolia Phenolics photosynthetic photon flux (PPF) |
本文献已被 SpringerLink 等数据库收录! |
|