The effect of extra bound cytochrome b-5 on cytochrome P-450-dependent enzyme activities in liver microsomes |
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Authors: | E G Hrycay R W Estabrook |
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Institution: | Department of Biochemistry The University of Texas Health Science Center Dallas, Texas 75235 USA |
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Abstract: | Binding of increasing amounts of detergent-purified cytochrome to rabbit liver microsomes produces a progressive inhibition of NADPH-cytochrome P-450 reductase activity which is accompanied by a similar inhibition of NADPH-supported benzphetamine demethylation. In contrast, NADH-cytochrome P-450 reductase activity in the enriched microsomes is markedly enhanced and this stimulation is accompanied by a similar increase in NADH-peroxidase activity, suggesting that cytochrome in these two reactions functions as an intermediate electron carrier to cytochrome P-450. |
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