局灶缺血性脑卒中大鼠侧脑室下带Shh信号通路成分的动态表达

通过研究Sonic hedgehog(Shh)信号通路成分在局灶缺血性脑卒中大鼠侧脑室下带(subventricular zone,SVZ)的动态表达,初步探讨该通路在局灶性缺血性脑卒中后神经再生的调控作用.将84只健康成年雄性SD大鼠随机分为正常组(n=12)、假手术组(n=12)、缺血6、12、24 h和3、7 d,共7组(n=12).采用线栓法制备大鼠右侧大脑中动脉阻断(middle cerebral artery occlussion,MCAO)模型.分别应用逆转录聚合酶链反应(RT-PCR)、免疫组化、免疫印迹法检测局灶脑缺血大鼠侧脑室下带Shh、Gli1 mRNA和蛋白变化.与正常组比较,Shh、Gli1mRNA和蛋白在假手术组表达变化不明显(P>0.05),模型组6 h表达增高(P<0.01),24 h达峰值(P<0.01),3 d时接近正常水平(P>0.05),7 d表达又升高(P<0.01).缺血性脑卒中可以上调Shh信号通路成分在SVZ区的表达,提示Shh信号通路可能参与卒中后神经再生机制的调控.

Dynamic Changes of the Expression of Sonic Hedgehog Signaling Pathway in the Subventricular Zone After Focal Cerebral Ischemia in Rats

To study the expression changes of Shh signaling pathway in the subventricular zone(SVZ) at dif-ferent times after focal cerebral ischemia and find out whether the Shh pathway is involved in the regulation of the differentiation of the proliferated neural precursor cells in the SVZ under ischemic conditions.Eighty-four SD rats were divided into normal control group,sham operation group,experimental group at 6,12,24 h and 3,7 d(n=12 for time point) after surgery.Experimental group was made by filament occlus...