Studies on the stimulation of gluconeogenesis and lipolysis by glucagon and epinephrine in isolated rat Kupffer cells.

Kupffer cells were isolated by collagenease-pronase treatment. Activity and leakage of GOT, GPT, LDH, GlDH and of nucleotide pyrophosphatase were measured and compared to parenchymal cells. In addition, the effects of glucagon and epinephrine on gluconeogenesis and lipolysis were studied. Both glucagon and epinephrine stimulated gluconeogenesis from lactate and alanine. The epinephrine response, however, was far greater than that of glucagon. Additional studies showed a 50% stimulation of lipolysis by epinephrine with triolein and tripalmitin as substrates. No stimulation of lipolysis was observed with glucagon.