A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13. |
| |
| Authors: | P H Schreier R Cortese |
| |
| Institution: | 1. Medical Research Council Laboratory of Molecular Biology Hills Road, Cambridge, CB2 2QH, England;2. Institut für Genetik der Universität zu Köln Weyertal 121, D 5000 Köln 41, West Germany |
| |
| Abstract: | The chain termination DNA sequencing procedure of Sanger et al. (1977) requires single-stranded DNA as template. M13 phage DNA exists as a single strand and therefore every DNA sequence cloned in M13 can be easily obtained in this form. Here we show that M13 single-stranded DNA pure enough to be used as a template for sequence determination can be prepared by simple centrifugation of the phage particle and extraction with phenol. |
| |
| Keywords: | dd used for 2′ 3′-dideoxy derivatives ddATP 2′ 2′-dideoxyadenosine-5′-triphosphate) |
| 本文献已被 ScienceDirect 等数据库收录！ |
