Juvenile hormone (JH) esterase activity but not JH epoxide hydrolase activity is downregulated in larval Adoxophyes honmai following nucleopolyhedroviruses infection |
| |
| Institution: | 1. Rubius Therapeutics, Cambridge, Massachusetts, USA. Past Chair, International Society for Cellular Therapy (ISCT) Commercialization Committee 2010–2014;2. Cell Medica Inc., Houston, Texas, USA. Immediate Past ISCT President, 2012–2014, and Chair, ISCT Advisory Board, 2014–2016;3. Division of Oncology, Laboratory of Cellular Therapy, University of Modena and Reggio Emilia, Modena, Italy. ISCT President 2014–2016, and Chair of the ISCT Presidential Task Force on the Use of Unproven Cellular Therapies;4. TxCell SA, Valbonne—Sophia Antipolis, France. Chair, ISCT Commercialization Committee 2014–2016;1. University of North Carolina Lineberger Comprehensive Cancer Center, Advanced Cellular Therapeutics Facility, Chapel Hill, North Carolina, USA. ISCT North America, Past Regional Vice President 2012–2014;2. Moffitt Cancer Center and Research Institute, Tampa, Florida, USA. Chair, ISCT NA LRA Committee, 2014–2016 and Co-Editor of the Telegraft;3. St Jude Children''s Research Hospital, Memphis, Tennessee, USA. ISCT Past Co-Chair, NA LRA Committee 2011–2014. ISCT North America, Regional Vice President 2014–2016;4. Division of Hematology, Cell Therapy Laboratory, The Ohio State University, Columbus, Ohio, USA. ISCT Global Secretary, 2013–2016 and Editor in Chief of Telegraft;1. Division of Hematology, Cell Therapy Laboratory, The Ohio State University, Columbus, Ohio, USA, International Society for Cellular Therapy (ISCT) Global Secretary 2013–2016, Editor in Chief of Telegraft;2. University of Minnesota Center for Bioethics and School of Public Health, Minneapolis, Minnesota, USA, Member at Large of the ISCT Presidential Task Force on the Use of Unproven Cellular Therapies;3. School of Public Policy, Georgia Institute of Technology, Atlanta, Georgia, USA, Member at large of the ISCT Presidential Task Force on the Use of Unproven Cellular Therapies |
| |
| Abstract: | Juvenile hormones (JHs) and ecdysteroids are critical insect developmental hormones. JH esterase (JHE) and JH epoxide hydrolase (JHEH) are JH-selective enzymes that metabolize JH and thus regulate the titer of JH. Baculoviruses are known to alter host endocrine regulation. The nucleopolyhedroviruses, AdhoNPV and AdorNPV, are known to have slow and fast killing activity against Adoxophyes honmai (Lepidoptera: Tortricidae), respectively. Here we found that when penultimate (4th) instar A. honmai are inoculated with AdhoNPV or AdorNPV, the mean survival time is 9.7 and 8.2 days, respectively. The larvae molted once but did not pupate. The AdhoNPV- or AdorNPV-infected larvae did not show a dramatic increase in JHE activity as was found in mock-infected larvae, instead they showed a marked decrease in JHE activity. In contrast, both viral infections had no effect on JHEH activity. In order to further characterize the JHE activity, the JHE-coding sequence of A. honmai (ahjhe) was cloned and confirmed to encode a biologically active JHE. Quantitative real-time PCR analysis of ahjhe expression in 4th and 5th instar A. honmai revealed that AdhoNPV and AdorNPV are able to reduce ahjhe expression levels. |
| |
| Keywords: | Juvenile hormone esterase (JHE) JH epoxide hydrolase (JHEH) Baculovirus Nucleopolyhedrovirus qRT-PCR |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
