A novel cytochrome P450 CYP6AB14 gene in Spodoptera litura (Lepidoptera: Noctuidae) and its potential role in plant allelochemical detoxification |
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| Affiliation: | 1. Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China;2. College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China;3. Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA;1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China;2. College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea |
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| Abstract: | Cytochrome P450 monooxygenases (P450s) play a prominent role in the adaptation of insects to host plant chemical defenses. To investigate the potential role of P450s in adaptation of the lepidopteran pest Spodoptera litura to host plant allelochemicals, an expressed sequence data set derived from 6th instar midgut tissues was first mined. One sequence identified from the S. litura 6th instar midgut EST database was determined by phylogenetic analysis to belong to the CYP6AB P450 subfamily, and named CYP6AB14. Dietary supplementation of S. litura larvae with either xanthotoxin (XAN), coumarin (COU) and flavone (FLA) led to elevated CYP6AB14 transcript levels in both midgut and fat body tissues. Injection of CYP6AB14-derived double-stranded RNA (dsRNA) into S. litura individuals significantly reduced CYP6AB14 transcript levels, and resulted in increased developmental abnormalities and higher mortality rates among XAN, COU and FLA-fed larvae. Our results strongly suggest a key role for CYP6AB14 in plant allelochemical detoxification in S. litura. |
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| Keywords: | Cytochrome P450 Plant allelochemical RNA interference |
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