IAA-induced apogamy in Platycerium coronarium (Koenig) Desv. gametophytes cultured in vitro |
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| Authors: | Siew-Hwa Kwa Yeow-Chin Wee Tit-Meng Lim Prakash P Kumar |
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| Institution: | (1) Department of Botany, National University of Singapore, Lower Kent Ridge Road, 0511 Singapore;(2) Department of Zoology, National University of Singapore, Lower Kent Ridge Road, 0511 Singapore |
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| Abstract: | Apogamous sporophytes were produced on Platycerium coronarium gametophytes cultured in the presence of indole-3-acetic acid (IAA). The percentage of apogamy as well as the total number of apogamous sporophytes produced per gametophyte clump were highest in the presence of 40  M IAA. When ethylene was allowed to accumulate in the culture vessel in the presence of an optimum level of IAA, the percentage and total number of apogamous sporophyte production decreased significantly. Using light microscope and confocal laser scanning microscope we have shown that nuclear size can be used as a quick parameter to estimate the ploidy level of P. coronarium.Abbreviations CLSM
confocal laser scanning microscope
- IAA
Indole-3-acetic acid
- MS
Murashige and Skoog |
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