Quantitative studies on RNA accumulation in human PHA-stimulated lymphocytes during blast transformation |
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| Authors: | L Srn P Biberfeld |
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| Institution: | 1. Department of Radiobiology, Research Institute of the Swedish National Defence, Department 1, 172 04 Sundbyberg 4, Sweden;2. Institute of Medical Cell Research and Genetics, Medical Nobel Institute, Karolinska Institutet, 104 01 Stockholm 60, Sweden;3. Department of Pathology, Karolinska Institutet, 104 01 Stockholm 60, Sweden |
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| Abstract: | The net RNA accumulation in PHA-stimulated individual lymphocytes during blast transformation was assayed. The RNA content of the cells was measured by UV microspectrophotometry and the concentration of ribosomes in the cytoplasm of non-stimulated and transforming lymphocytes was determined by counting RNP particles in electronmicrographs.The mean RNA content of non-stimulated lymphocytes was 1.9 ± 0.8 × 10−12g/cell. During transformation the RNA content increased considerably, the maximal increase being about ten-fold. This increase was proportional to the increase in cellular dry mass. In fact, the RNA concentration was constant during transformation and approximately the same in non-stimulated and transforming lymphocytes. Furthermore the ribosome concentration was approximately the same in the cytoplasm of non-stimulated and transforming lymphocytes. However, a pronounced transition of single ribosomes to ribosomal clusters occurred during transformation and was one of the earliest structural signs of stimulation observed. |
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