Restriction fragment length polymorphisms for growth hormone, prolactin, osteonectin, α crystallin, γ crystallin, fibronectin and 21-steroid hydroxylase in cattle |
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| Authors: | J. L. THEILMANN,L. C. SKOW,J. F. BAKER&dagger ,J. E. WOMACK |
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| Affiliation: | Departments of Veterinary Pathology, Texas A&M University and the Texas Agricultural Experiment Station, College Station, Texas, USA;*Veterinary Anatomy, Texas A&M University and the Texas Agricultural Experiment Station, College Station, Texas, USA;†Animal Science, Texas A&M University and the Texas Agricultural Experiment Station, College Station, Texas, USA |
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| Abstract: | Summary. Genomic DNAs from animals representing six breeds of cattle (Angus, Brahman, Hereford, Holstein, Jersey and Texas Longhorn) were screened with cloned gene probes in a search for restriction fragment length polymorphisms (RFLPs). Eleven RFLPs were identified using seven different probes: growth hormone, prolactin, osteonectin, α A-crystallin, γ crystallin, fibronectin and 21-steroid hydroxylase. The frequencies of the alleles identified by each probe were calculated and compared in a limited sampling of the six bovine breeds. These polymorphisms greatly enhance the pool of immunogenetic, biochemical and molecular markers available in cattle for linkage analysis, testing of parentage, and distinction of breeds. |
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| Keywords: | RFLP polymorphisms DNA cattle growth hormone prolactin osteonectin α crystallin γ crystallin fibronectin 21-steroid hydroxylase |
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