Effect of S-100 protein on assembly of brain microtubule proteins in vitro |
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Authors: | R Donato |
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Affiliation: | Department of Biochemistry, King''s College London, Strand, London, WC2R 2LS, England |
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Abstract: | S-100 protein inhibits the assembly of brain microtubule proteins in vitro in the presence of 10 microM free Ca2+. The S-100 effect is generally greater on the rate than on the extent of assembly, and even greater as the microtubule protein concentration decreases and the time of preincubation between S-100 and microtubule proteins before GTP addition increases, at a given S-100/tubulin dimer molar ratio. The S-100 effect is greatly enhanced in the presence of physiological concentrations of K+ and is completely reversed by EGTA. |
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Keywords: | Superoxide Hydroxyl radical Iron Haber—Weiss reaction Chelating agent Fenton reaction |
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