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Sake brewing characteristics of a new type of cerulenin-resistant sake yeast mutant
Authors:Hisashi Fukuda  Yasuzo Kizaki  Takeaki Ishikawa  Koujiro Takahashi
Affiliation:(1) National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima, 739-0046, Japan;(2) National Research Institute of Brewing, 2-6-30 Takinogawa, Kitaku, 114-0023, Japan;(3) Brewing Society of Japan, 2-6-30 Takinogawa, Kitaku, 114-0023, Japan;(4) Takara Shuzo Company, Ltd, Shijo-karasuma Higashiiru Shimogyo-ku, Kyoto, 600-8688, Japan
Abstract:Several new types of cerulenin-resistant mutants of sake yeast were isolated. These mutants showed respiratory deficiency and could grow on media containing a higher concentration of antibiotics than could the parent. Sakes brewed by the mutants produced less succinate than by both the parent yeast and the mutants with respiratory deficiency induced by ethidium bromide. In addition, the acidity of these mutants was decreased. Since low acidity is favourable in both sake and wine, these mutants might be applicable for both sake and wine brewing.
Keywords:cerulenin  cycloheximide respiratory deficiency  Saccharomyces cerevisiae  sake
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