The molecular genetic differentiation of cultured <Emphasis Type="Italic">Saccharomyces</Emphasis> strains |
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| Authors: | E?S?Naumova M?V?Zholudeva N?N?Martynenko Email author" target="_blank">G?I?NaumovEmail author |
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| Institution: | (1) State Research Institute for Genetics and Selection of Industrial Microorganisms (GosNIIGenetika), Pervyi Dorozhnyi proezd 1, Moscow, 117545, Russia;(2) State University of Foodstuffs, Moscow, Russia |
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| Abstract: | A comparative molecular genetic study of cultured Saccharomyces strains isolated from the surface of berries and various fermentation processes showed that baker s yeast and black-currant isolates contain not only Saccharomyces cerevisiae but also S. cerevisiae × S. bayanus var. uvarum hybrids. The molecular karyotyping of bakers, brewers, and wine yeasts showed their polyploidy. The restriction enzyme analysis of noncoding rDNA regions (5.8S-ITS and IGS2) makes it possible to differentiate species of the genus Saccharomyces and to identify interspecies hybrids. The microsatellite primer (GTG)5 can be used to study the populations of cultured S. cerevisiae strains.__________Translated from Mikrobiologiya, Vol. 74, No. 2, 2005, pp. 215–223.Original Russian Text Copyright © 2005 by Naumova, Zholudeva, Martynenko, Naumov. |
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| Keywords: | Saccharomyces S cerevisiae × S bayanus var uvarum hybrids RFLP analysis rDNA molecular karyotyping |
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