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Assessment of DNA fingerprinting for determining genetic diversity in sheep populations
Authors:I F Hermans  G K Chambers  T W Jordan  C A Morris  N R Towers
Institution:School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand;AgResearch, Ruakura Agricultural Centre, Hamilton, New Zealand
Abstract:Genomic DNA, prepared from 12 animals from four sheep flocks, was digested with either HaeIII or Hin fI and probed with three DNA fingerprinting probes. Mean DNA fingerprint band sharing and band frequency calculated for each flock were used to estimate genetic diversity. Each of the DNA fingerprinting systems showed the same trend in diversity within the sampled flocks, and greater diversity between the flocks than within the flocks. DNA fingerprinting therefore provides a useful measure of genetic diversity in sheep.
Keywords:DNA  fingerprinting  polymorphism  genetic diversity  sheep breeds
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