宽叶韭种内分化的同工酶及可溶性蛋白的研究

采用聚丙烯酰胺凝胶电泳（ＰＡＧＥ）对宽叶韭的３个代表居群内不同植株间的过氧化物酶、酯酶和１６个居群间的酯酶（ＥＳＴ）、过氧化物酶（ＰＯＸ）、葡萄糖－６－磷酸脱氢酶（Ｇ６ＰＤＨ）、苹果酸酶（ＭＥ）、苹果酸脱氢酶（ＭＤＨ）、天冬氨酸转移酶（ＡＡＴ）、腺苷三磷酸酶（ＡＴＰａｓｅ）、乳酸脱氢酶（ＬＤＨ）８种同工酶系统以及可溶性蛋白进行了分析。结果发现：居群内不同植株的酶谱无差异；居群间的酶谱存在多态性，酶谱差异明显，且各居群均有其特征酶谱。采用“排序”方法和聚类分析，对宽叶韭１６个居群酶谱的相似系数和酶谱距离进行了研究。同工酶谱表型差异彼此显著的３个代表居群的可溶性蛋白双向电泳图谱也表现出明显差异，印证了同工酶的分析结果。结合细胞学的最新核型证据，从生物化学角度确定了这１６个居群间的亲缘关系和彼此的分化程度。结果表明：宽叶韭明显分为两大类，即冬季不倒苗和冬季倒苗两类，在同一类型中各居群之间彼此近缘，且冬季不倒苗的类型分化程度较高。本研究首次将多种同工酶的综合分析与聚类分析及可溶性蛋白双向电泳用于葱属植物的种内分化研究，为植物分子进化和系统学研究提供了科学依据

The study on isozyme and soluble proteins of intraspecies differentiation in Allium hookeri

In this paper, isozymes of POX, EST of bionts of 3 populations and POX, EST, G6PDH, ME MDH, AAT, ATPase and LDH of 16 populations of A hookeri and soluble proteins of 3 of the 16 populations were analysed by means of polyacrylamide gel electrophoresis The results indicate that the isozymes are non-different among the bionts of one population, the isozymes patterns and soluble proteins ones are remarkably different among the populations with each showing characteristic isozymes and soluble proteins pattern onthe basis of the Rf value and isozymes activity Moreover, the resemblance of the isozymes patterns of the 16 populations of A hookeri were analysed using the methods of arranging and clustering analysis The results indicate that perennial A hookeri, which include all three triploids populations, Pingshan, Ruyuan and Longsheng populations, and such diploids as Dafeishui,Emei Qingyinge, Muchuan, Qingshen, Wulong and Nanchuan populations studied in our research, are closely related among various populations within the same type, whereas non-perennial A hookeri populatins, which wither during the winter, are also closely related to each other within the same type Differentiation level of perennial populations is rather high The originality of this paper exists in the application of IEF/SDS-PAGE in the research of intraspecies differentiation of Allium, and our experiments prove that this research approach is more indicative and persuasive In conclusion, isozymes and soluble proteins can be genetic markers of the genetic differences and relationship among intraspecies And they provide reliable biochemical basis for the study of intraspecies differentiation of plant