Isotope effects on binding of NAD+ to lactate dehydrogenase |
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| Authors: | R D LaReau W Wan V E Anderson |
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| Institution: | Department of Chemistry, Brown University, Providence, Rhode Island 02912. |
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| Abstract: | The isotope effect on binding 4-2H]NAD+ and 4-3H]NAD+ to lactate dehydrogenase has been shown to be 1.10 +/- 0.03 by whole molecule isotope ratio mass spectrometry and 1.085 +/- 0.01 by 3H/14C scintillation counting. These values demonstrate that specific interactions of the nicotinamide ring with the enzyme make the C-H bond at C-4 less stiff in the binary complex. |
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