Photoregulation of germination in seed of transgenic lines of tobacco and Arabidopsis which express an introduced cDNA encoding phytochrome A or phytochrome B |
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Authors: | Alex C McCormac Harry Smith Garry C Whitelam |
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Institution: | (1) Department of Botany, University of Leicester, LE1 7RH Leicester, UK |
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Abstract: | Photoinduction and photoinhibition of germination in seed from a homozygous tobacco (Nicotiana tabacum L.) line containing an introduced oat phyA cDNA (encoding phytochrome A) is compared with that of isogenic wild-type (WT) tobacco. Under continuous irradiation by a light source with a low redfar-red (RFR) ratio the transgenic tobacco seed appeared to be less susceptible to photoinhibition of germination compared with WT seed. However, induction of germination following a short pulse by R (666 nm) was not enhanced in the genotype transformed by oat phyA cDNA compared with the WT; neither did germination of the transgenic tobacco seed show an increased sensitivity to saturating pulses of light of longer wavelengths (666–730 nm). In seeds of transgenic Arabidopsis thaliana (L.) Heynh. which contained an introduced phytochrome-B-encoding cDNA, levels of dark germination were enhanced, consistent with mediation of response by phytochrome B-Pfr. The germination behaviour of Arabidopsis genotypes wich contained an introduced cDNA encoding phytochrome A, however, did not significantly differ from that of the WT.Abbreviations ABO
seed transformed with Arabidopsis phyB
- cDNA; CaMV
cauliflower mosaic virus
- FR
far-red light
- Pfr
far-red-absorbing form of phytochrome
- Ptot
total phytochrome
- Pfr/Ptot
phytochrome photoequilibrium
- R
red light
- RBO
seed transformed with rice phyB cDNA
- RFR
quantum ratio of red and far-red light
- WL
white light
- WL + FR
whitelight supplemented with far-red light
- WT
wild type
The authors wish to thank R.D. Vierstra (Department of Horticulture, University of Wisconsin-Madison, USA) for providing the transgenic tobacco line, and M.T. Boylan, D. Wagner and P.H. Quail (U.C. Berkeley/USDA Plant Gene Expression Center, Albany, Calif. USA) for providing the transgenic Arabidopsis lines. The work presented in this paper was funded by grants from the Agricultural and Food Research Council (H.S., A.C.M., G.C.W.). |
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Keywords: | Arabidopsis (transgenic) Germination Light and germination Nicotiana (transgenic) Phytochrome A (phyA cDNA) Phytochrome B (phyB cDNA) |
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