Micropropagation of calla lily (<Emphasis Type="Italic">Zantedeschia albomaculata</Emphasis>) via <Emphasis Type="Italic">in vitro</Emphasis> shoot tip proliferation |
| |
Authors: | H?S?Chang D?Charkabarty E?J?Hahn Email author" target="_blank">K?Y?PaekEmail author |
| |
Institution: | (1) Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763 Cheong-ju, Korea |
| |
Abstract: | Summary A method for the micropropagation of Zantedeschia albomaculata is presented using shoot tip proliferation onto Murashige and Skoog (MS) medium supplemented with different plant growth
regulator concentrations and combinations. Of the four cytokinins tested, 6-benzyladenine (BA) and thidiazuron (TDZ) were
found to be more effective. An optimal concentration of BA (8.87 μM) or TDZ (4.54 μM) developed an average of 3.8 and 3.2 shoots per explant, respectively, but increasing concentrations of cytokinins often
led to lower proliferation rate and stunted growth. Addition of auxins to the MS medium supplemented with 8.87 μM BA slightly enhanced multiple shoot formation in the explants. Multiplication of six cultivars of Zantedeschia genus comprising different flower types and colors were tested and achieved using only one regeneration medium (MS+8.87 μMBA+2.46 μM IBA). Different MS medium strength, air temperature (15, 20, 25, and 30°C) and light quality fluorescent, red + blue, red
and blue light provided by a LED (light-emitting diode) system] were used (without phytohormone) with the aim of stimulating
in vitro shoot and root development. Half-strength MS or MS and cultures maintained at 25°C were found to be equally suitable for
shoot tip culture of Z. albomaculata. Shoot elongation as well as fresh and dry weight were significantly increased when cultures were kept under red or blue
light. |
| |
Keywords: | LEDs (light-emitting diodes) mass propagation calla lily Zantedeschia albomaculata light quality |
本文献已被 SpringerLink 等数据库收录! |
|