| HPLC法测定杨梅叶中杨梅苷的含量 |
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| 引用本文： | 齐洁,万竹青,李默影,吴秋月,刘丽芳. HPLC法测定杨梅叶中杨梅苷的含量[J]. 中国野生植物资源, 2012, 31(1): 35-37 |
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| 作者姓名： | 齐洁 万竹青 李默影 吴秋月 刘丽芳 |
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| 作者单位： | 中国药科大学天然药物活性物质与功能国家重点实验室,江苏南京,210009 |
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| 摘 要： | 目的:建立HPLC法测定杨梅叶中杨梅苷含量的方法。方法:采用Diamonsil C18(150 mm×4.6 mm,5μm)柱,流动相乙腈-0.1%磷酸水溶液梯度洗脱,流速1.0 mL/min,检测波长358 nm,柱温30℃。结果:杨梅苷在0.088-0.880 mg/mL内与峰面积呈良好线性关系,A=36113C-401.56,r=0.9994;平均回收率为102.4%,RSD=1.9%(n=9)。测得杨梅叶中杨梅苷含量为1.43%。结论:该测定方法准确,可靠,对于杨梅的进一步开发利用具有重要的参考意义。
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| 关 键 词： | 高效液相色谱法 杨梅叶 杨梅苷 |
Determination of Myricitrin in Leaves of Myrica rubra Sieb. et Zucc. by HPLC |
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| Qi Jie , Wan Zhuqing , Li Moying , Wu Qiuyue , Liu Lifang. Determination of Myricitrin in Leaves of Myrica rubra Sieb. et Zucc. by HPLC[J]. Chinese Wild Plant Resources, 2012, 31(1): 35-37 |
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| Authors: | Qi Jie Wan Zhuqing Li Moying Wu Qiuyue Liu Lifang |
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| Affiliation: | (State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009,China) |
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| Abstract: | Objective: To develop an HPLC method for determination of myricitrin in leaves of Myrica rubra Sieb.et Zucc..Methods: The samples were separated on a Diamonsil C18(150 mm×4.6 mm,5 μm) column with the mobile phase of acetonitrile-0.1% phosphoric acid(gradient elution).The flow rate was 1.0mL/min.The detection wavelength was 358 nm.The column temperature was 30 ℃.Results: The linear range of myricitrin was 0.088-0.880mg/mL,A=36113C-401.56,r=0.9994.The average recovery was 102.4%,RSD=1.9%(n=9).The content of myricitrin in leaves of Myrica rubra Sieb.et Zucc.was 1.43%.Conclusion: The method was accurate and reliable,which is useful for the further exploring of this plant. |
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| Keywords: | HPLC leaves of Myrica rubra Sieb.et Zucc. myricitrin |
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