Techniques for the optimum recovery of cold injured Campylobacter jejuni from milk or water |
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Authors: | T. J. Humphrey |
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Affiliation: | Area Public Health Laboratory, Church Lane, Heavitree, Exeter EX2 5AD, UK |
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Abstract: | When broth was inoculated with cells of Campylobacter jejuni that had been injured by chilling there was a fall in the viable population of up to 90%. It was greater at 43° than 37°C and in the presence of certain antibiotics and in some cases resulted in a surviving population that was below the minimum inoculum for subsequent growth. A technique of pre-enrichment in non-selective culture broth at 37°C for 2 h before the addition of antibiotics and incubation at 43°C was found to significantly reduce the fall in numbers and to improve the detection of C. jejuni in samples of raw milk and water. |
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