健康人口腔中EB病毒排出情况的研究

本研究对30名血清EB病毒抗体阳性健康人从口腔排出EB病毒的情况,作了15个月的反复观察。发现28人有病毒排出,其中每次检查均为阳性者6人,间断阳性者22人,有2人多次检查均为阴性。这一结果提示,EB病毒可能是通过它在口咽部某些特定细胞内的慢性复制而在活体内持续存在的。

OROPHARYNGEAL SHEDDING OF INFECTIOUS EPS- TEIN-BARR VIRUS IN HEALTHY VIRUS IMMUNE DONORS

A most sensitive method has been developed for the detection of Epstein-Barr ( EB ) virus in throat washings.Cryopreserved preparations of foetal cord blood lymphocytes, from samples already found to be optimally sensitive to virus-induced transformation in vitro, were exposed to concentrates prepared from throat washings by ultracentrifugation, the cells were then cultured for 8 weeks to observe the incidence of EB virus-induced transformation to lymp-hoblastoid cell lines.Using this method, oropharyngeal shedding of EB virus has been monitored in a prospective study involving 30 healthy adults (all but one of Caucasian origin ) with serological evidence of previous EB virus infection.Of these individuals, 6 shed virus on every occasion of testing over a period of 15 months, 13 shed virus on most occasions, 9 shed virus on less than half the occasions of testing, whilst only 2 never gave evidence of virus. Shedding.This work indicates that the extent of EB virus shedding in healthy seropositive individuals is very much greater than what previous reports in the literature have suggested, and raises the possibility that EB virus may actually persist in vivo via chronic,usually low-grade, replication in a permissive cell type in the cropharynx.