A Cyanobacterial Gene Encoding Peptidyl-Prolyl cis-trans Isomerase |
| |
Authors: | Hassidim M Schwarz R Lieman-Hurwitz J Marco E Ronen-Tarazi M Kaplan A |
| |
Affiliation: | Department of Botany, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel. |
| |
Abstract: | The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not able to isolate a kanamycin-resistant mutant in which all the genomic wild-type copies were substituted, which suggests that such replacement could have been lethal. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|