A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction |
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| Authors: | Hervé Pelloux,Judy Weiss,Josiane Simon,Franç ois Muet,hé lè ne Fricker-hidalgo,ré e Goullier-fleuret,Pierre Ambroise-thomas |
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| Affiliation: | Département de Parasitologie-Mycologie Médicale et Moléculaire, Facultéde Médecine, UniversitéJoseph Fourier, 38043 Grenoble, France; Roche Molecular Systems, 1145 Atlantíc Ave., Alameda, CA 94501, USA; Service de Gynécologie-Obstétrique et Médecine de la Reproduction, Centre Hospitalier Universitaire de Grenoble, 38043 Grenoble, France |
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| Abstract: | Abstract A new PCR system including a pair of primers, a probe and an internal control were designed from the B1 gene of Toxoplasma gondii . The system described allowed the detection of less than 10 tachyzoites of the RH strain of T. gondii . Among 21 amniotic fluid samples, this system diagnosed the cases of congenital toxoplasmosis which were simultaneously diagnosed using mice inoculation, in vitro culture, and serology from both amniotic fluid and fetal blood. These results show that these new primers allow for a highly sensitive detection of T. gondii DNA. |
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| Keywords: | Toxoplasma gondii PCR Amniotic fluid Diagnosis |
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