Dental pulp collagenase: initial demonstration and characterization. |
| |
Authors: | J Kishi K Iijima T Hayakawa |
| |
Institution: | Department of Biochemistry, School of Dentistry Aichi-Gakuin University, 2-11 Suemori-dori Chikusa-ku, Nagoya 464, Japan |
| |
Abstract: | A collagenase, active against native helical collagen, was initially found in the explant medium of bovine dental pulp. In contrast to the collagenases from other oral tissues, all the pulp enzyme released was in a latent form which was activated by trypsin treatment, 4-aminophenylmercuric acetate, and some chaotropic agents. The activated enzyme was inhibited by low concentrations of EDTA and calf serum. The molecular weight of activated enzyme was tentatively estimated at 45,000 daltons by gel filtration. The enzyme attacked undenatured collagen in solution at 20°C producing characteristic products and . |
| |
Keywords: | 4-APMA 4-aminophenylmercuric acetate SDS sodium dodecyl sulfate |
本文献已被 ScienceDirect 等数据库收录! |
|