Wright's Stain for Chloroplasts and Tissue Spreads

Wright's stain, including the entire Wright method as used in hematology, is applicable to fresh animal and plant tissues which may be spread with rapid drying. Particularly are the chloroplasts and vacuole plastids of Nitella well prepared by: pricking open a blotted, uninjured (streaming) cell upon a dry slide, thus bursting out both chloroplasts and other plastids which spread themselves; further teasing of more plastids from the cell membrane upon an adjacent dry region of the slide. Also Tunicate zooids blotted dry in transference to a slide may be torn open and the various tissues teased and lifted about on the slide with excellent spreading of cells. Rapid drying with gentle heat, well timed fixing and staining and differentiation all contribute to results which not only show normal cellular differentiations but which are very valuable in showing experimental cytological changes.