The assembly of tubulin into membranes |
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Authors: | Howard Feit Jerry W Shay |
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Institution: | Departments of Neurology and Cell Biology, The University of Texas Health Science Center, 5323 Harry Hines Blvd., Dallas, Texas 75235, USA |
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Abstract: | Tubulin in high-speed supernatants of brain undergoes an alternate form of polymerization into structures that resemble membranes rather than microtubules. The reaction required elevated temperature (37°C) and was prevented by 1mM CaCl2 or 10?4 M maytansine. The membraneous material was composed of tubulin (80%) and microtubule-associated proteins (8%) and contained phospholipids. The tubulin was identified on the basis of comigration in two-dimensional gel electrophoresis and colchicine-binding activity. |
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