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Three‐dimensional rendering of otolith growth using phase contrast synchrotron tomography
Authors:J J I Mapp  M H Fisher  R C Atwood  G D Bell  M K Greco  S Songer  E Hunter
Institution:1. School of Computing Sciences, University of East Anglia, Norwich, U.K.;2. Diamond Light Source Ltd., Didcot, Oxfordshire, U.K.;3. Faculty of Health and Science, University Campus Suffolk, Ipswich, U.K.;4. Faculty of Science, School of Dentistry and Health Sciences, Charles Sturt University, Bathurst, NSW, Australia;5. University of Bath Bee Unit, Department of Biology and Biochemistry, Faculty of Sciences, University of Bath, U.K.;6. Centre for Environment, Fisheries and Aquaculture Science, Lowestoft, Suffolk, U.K.
Abstract:A three‐dimensional computer reconstruction of a plaice Pleuronectes platessa otolith is presented from data acquired by the Diamond Light synchrotron, beamline I12, X‐ray source, a high energy (53–150 keV) source particularly well suited to the study of dense objects. The data allowed non‐destructive rendering of otolith structure, and for the first time allows otolith annuli (internal ring structures) to be analysed in X‐ray tomographic images.
Keywords:annuli  fish ageing  non‐destructive sampling
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