| 絮凝剂BP25的化学组成及结构研究 |
| |
| 引用本文： | 刘紫鹃 徐桂云 刘志培 杨惠芳. 絮凝剂BP25的化学组成及结构研究[J]. 微生物学报, 2001, 41(3): 348-352 |
| |
| 作者姓名： | 刘紫鹃 徐桂云 刘志培 杨惠芳 |
| |
| 作者单位： | 1. 中国科学院微生物研究所
2. 中国科学院化学研究所 |
| |
| 基金项目： | 国家"九五”攻关项目(96-909-01-03) |
| |
| 摘 要： | 从活性污泥里筛选到一株巨大芽孢杆菌 (Bacillusmegaterium)A2 5,它能分泌胞外絮凝剂。用乙醇沉淀及SephadexS 50 0分子筛层析得到絮凝剂纯品BP2 5。通过Bradford反应、琼脂糖凝胶电泳及硫酸 酚法测定糖 ,证明BP2 5是一类多糖类物质。应用核磁共振技术证明其不含有糖醛酸。经三甲基硅醚和甲基化的气相色谱 质谱分析 ,证明多糖BP2 5含有葡萄糖和甘露糖两种单糖 ,其摩尔比为 4∶1。其连接键型包括α 1 ,6糖苷键和α 1 ,3糖苷键。由此推导出BP2 5可能的单元结构。
|
| 关 键 词： | 絮凝剂 多糖 甲基化分析 色谱质谱联用 |
| 文章编号： | 0001-6209(2001)03-0348-05 |
| 修稿时间： | 2000-04-21 |
COMPOSITION AND STRUCTURE OF BIOFLOCCULANT BP25 |
| |
| Z Liu,G Xu,Z Liu,H Yang. COMPOSITION AND STRUCTURE OF BIOFLOCCULANT BP25[J]. Acta microbiologica Sinica, 2001, 41(3): 348-352 |
| |
| Authors: | Z Liu G Xu Z Liu H Yang |
| |
| Affiliation: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. |
| |
| Abstract: | A strain of Bacillus megaterium screened from activated sludge could produce exocellular flocculant. The flocculant was purified through ethanol precipitation and Sephadex S-500 column chromatography. The purified flocculant BP25 was assayed by Bradford reaction, agarose gel electrophoresis and Sulfate-phenol method. The results showed that BP25 was a kind of polysaccharide which contains 36.97% O, 6.28% H, 47.00% C, 0% No NMR assay showed BP-25 contains no uronic acid. Gas-chromatography assay combined with thin-layer chromatography of acid hydralate revealed that BP25 contains Glucose and Mannose with the mole ratio of 4:1. Methylation analysis revealed the polysaccharide contains alpha-1, 6 glycosidicbond and alpha-1, 3 glycosidicbond. The main chains are comprised of Glucose and all the Mannose are in the side chains. Possible repeating unit structure was deduced. |
| |
| Keywords: | Flocculant Polysaccharide Methylation analysis GC/MS |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
|
点击此处可从《微生物学报》下载全文 |
|
