| 微碳纤电极与细胞量子化分泌的记录方法 |
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| 引用本文： | 何立铭,段开来,张晨,何林玲,熊巍,李洁,周专. 微碳纤电极与细胞量子化分泌的记录方法[J]. 生物物理学报, 2002, 18(2): 147-155 |
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| 作者姓名： | 何立铭 段开来 张晨 何林玲 熊巍 李洁 周专 |
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| 作者单位： | 1. 中国科学院神经科学研究所,上海,200031;华中理工大学生物物理与生物化学研究所,湖北,武汉,430074
2. 中国科学院神经科学研究所,上海,200031 |
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| 基金项目： | 国家自然科学基金 (39525009，39970238，39970371)，中国科学院百人计划，《国家重点基础研究发展规划》(G2000077800)资助 |
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| 摘 要： | 电生理研究细胞分泌有两种实时记录技术。膜电容法通过记录细胞表面积来估算刺激诱导的细胞分泌和胞吞导致的膜表面积总和变化。微碳纤电极（carbon fiber electrode,CFE )法则可单纯记录细胞分泌。CFE记录灵敏度是当前最高水平，可记录单个50nm突触小泡的量子递质释放。介绍CFE基本记录方法、原理和一些实验应用。
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| 关 键 词： | 微碳纤电极 细胞量子化分泌 记录方法 细胞分泌 膜片钳 神经递质 膜电容 |
| 文章编号： | 1000-6737(2002)02-0147-09 |
| 修稿时间： | 2001-11-08 |
MICRO CARBON FIBER ELECTRODE FOR RECORDING OF QUANTAL SECRETION IN SINGLE CELL |
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| HE Li-ming,,DUAN Kai-lai,ZHANG Chen,HE Lin-ling,XIONG Wei,LI Jie,ZHOU Zhuan. MICRO CARBON FIBER ELECTRODE FOR RECORDING OF QUANTAL SECRETION IN SINGLE CELL[J]. Acta Biophysica Sinica, 2002, 18(2): 147-155 |
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| Authors: | HE Li-ming DUAN Kai-lai ZHANG Chen HE Lin-ling XIONG Wei LI Jie ZHOU Zhuan |
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| Affiliation: | HE Li-ming1,2,DUAN Kai-lai1,ZHANG Chen1,HE Lin-ling1,XIONG Wei1,LI Jie1,ZHOU Zhuan1,2 |
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| Abstract: | Biophysical technologies for single cell recording was described, with special focus on micro carbon fiber electrode (CFE) and its application in stimulus-secretion coupling. In contrast to membrane capacitance of patch-clamp recording, CFE has advantages in high sensitivity, real detection of release substance and no need for tight-seal patch-clamp, although it detects only a few easily oxidizable transmitters including catecholamines and serotonin. In combination with patch-clamping and Ca imaging measurement, coupling between action-potentials and quantal transmitter secretion in chromaffin cells are demonstrated as the example of CFE application. |
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| Keywords: | Secretion Patch-clamp Neurotransmitter Membrane capacitance Carbon fiber electrode |
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