Chi18A,the endochitinase in the cellulosome of the thermophilic,cellulolytic bacterium Clostridium thermocellum |
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Authors: | Zverlov Vladimir V Fuchs Klaus-Peter Schwarz Wolfgang H |
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Institution: | Institute for Molecular Genetics, Russian Academy of Science, 123 187 Moscow, Russia. |
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Abstract: | The chitinase gene chiA was identified on the Clostridium thermocellum genome downstream of the endoglucanase gene celA. It contains a catalytic module of glycosyl hydrolase family 18 and a cellulosomal dockerin module. Chi18A hydrolyzes aryl-acetyl-chito-oligosaccharides preferentially. In denaturing electrophoresis of purified cellulosomes, a single chitinase activity band was identified in zymograms and Western blots, indicating that Chi18A is the only chitinase in the cellulosome. |
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