Propagation of CAR bacillus in artificial media. |
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| Authors: | Y Shoji T Itoh N Kagiyama |
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| Affiliation: | Central Institute for Experimental Animals, Kanagawa, Japan. |
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| Abstract: | CAR bacillus propagated successfully in an artificial medium, and the number of CAR bacillus was about 30 times the original number after 8 days of cultivation. The medium consisted of Eagle's minimum essential medium supplemented with 10% fetal calf serum and 20% hamster tracheal organ culture soup. By intranasal inoculation to mice, two strains of the CAR bacillus passaged 5 and 6 times in this artificial medium produced the same lung lesions as natural CAR bacillus infection. |
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