Effects of selected vasoactive substances on adenylate cyclase activity in brain,isolated brain microvessels,and primary cultures of brain microvessel endothelial cells |
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Authors: | Anna Baranczyk-Kuzma Kenneth L Audus François L Guillot Ronald T Borchardt |
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Institution: | (1) Department of Biochemistry Institute of Biopharmacy, Warsaw Medical School, Banacha 1, 02-097 Warsaw, Poland;(2) Department of Pharmaceutical Chemistry, The University of Kansas, School of Pharmacy, 66045 Lawrence, Kansas;(3) Biopharmaceutical Department, Sandoz Ltd., 4002 Basel, Switzerland |
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Abstract: | The specific activity of adenylate cyclase was assayed in homogenates of gray matter, freshly isolated and primary cultured microvessel endothelial cells from bovine cerebral cortex. Specific activities for the tissues were 14.6±2.1, 15.6±2.7, and 8.4±1.5 pmol cAMP/mg protein/min±SD for gray matter, cultured microvessels, and freshly isolated microvessels, respectively. Adenylate cyclase associated with gray matter and cultured microvessels was sensitive to histamine and selected catecholamines. Perhaps due to metabolic deficiencies, adenylate cyclase of freshly isolated microvessels exhibited little or no response to either the catecholamines or histamine. Angiotensin II stimulated adenylate cyclase of both freshly isolated and cultured microvessels but had no effect on gray matter. Bradykinin did not stimulate cAMP generation in any of the tissues. Overall results support the role of cAMP in regulating brain microvessel functions and suggest that primary cultures of brain microvessels may be useful in examining cAMP-mediated biochemical pathways at the blood-brain barrier. |
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Keywords: | brain microvessel endothelia blood-brain barrier adenylate cyclase cAMP angiotensin II histamine catecholamines bradykinin |
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