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Transgenic roots produced by introducing Ri-rol genes into cucumber cotyledons by particle bombardment |
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| Authors: | Hiroaki Kodama Kohei Irifune Hiroshi Kamada Hiromichi Morikawa |
| Institution: | (1) Applied Microbiology Department, Nishiki Research Laboratories, Kureha Chemical Industry Co., Ltd., Ochiai, 16, Nishiki, 974 Iwaki, Fukushima, Japan;(2) Department of Biological Sciences, Faculty of Science, Hiroshima University, 724 Higashi-Hiroshima, Japan;(3) Gene Experiment Center, Institute of Biological Sciences, University of Tsukuba, 305 Tsukuba, Ibaraki, Japan;(4) Present address: Department of Biology, Faculty of Science, Kyushi University, Higashiku, 812 Fukuoka, Japan |
| Abstract: | Transformed roots ofCucumis sativus were obtained from cotyledon tissues that had been bombarded with gold particles coated with plasmid pE7.4 using a pneumatic particle gun. This plasmid containsrolA, rolB, rolC genes and ORF 13 of the 7.4 kbEco RI fragment of T-DNA of pRi 1724 isolated fromAgrobacterium rhizogenes MAF 03-01724. The nature of the tissue and the composition of the culture media used greatly influenced the recovery of transformed roots. The transgenic nature of the derived roots was confirmed by the vigorous. highly-branched growth seen on a phytohormone-free medium. The stable integration ofrol genes into the cucumber genome was confirmed by Southern blot analysis. |
| Keywords: | rol genes transgenic root Cucumis sativus particle bombardment hairy root |
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