High concentration cultivation of Bifidobacterium longum in fermenter with cross-flow filtration |
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Authors: | Masayuki Taniguchi Nobuharu Kotani Takeshi Kobayashi |
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Institution: | (1) Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464 Nagoya, Japan;(2) Present address: Department of Chemical Engineering, Faculty of Engineering, Niigata University, Japan |
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Abstract: | Summary High concentration cultivation of Bifidobacterium longum in a fermenter with cross-flow filtration using a ceramic filter is described. Continuous cross-flow filtration allowed complete recycling of the cells to the fermenter and also continuous separation of inhibitory metabolites. The final cell concentration attained in the cultivation was 54.4 g dry wt./l; this was seven times as high as that without cross-flow filtration. The time course of the cultivation with cross-flow filtration was predicted, based on the assumption that the specific growth rate can be expressed only as a function of concentrations of metabolites (acetate and lactate) in a culture broth.Nomenclature
D
dilution rate (h-1)
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m
maintenance coefficient (h-1)
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OD
570
optimal density at 570 nm
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P
A
acetate concentration (g/l)
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P
A0
initial acetate concentration (g/l)
-
P
L
lactate concentration (g/l)
-
P
L0
initial lactate concentration (g/l)
-
S
lactose (substrate) concentration (g/l)
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S
0
initial lactose (substrate) concentration (g/l)
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t
cultivation time (h)
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Y
x/s
growth yield (g/g)
-
X
dry cell concentration (g/l)
-
X
0
initial dry cell concentration (g/l)
-
constant
-
constant |
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Keywords: | |
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