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High concentration cultivation of Bifidobacterium longum in fermenter with cross-flow filtration
Authors:Masayuki Taniguchi  Nobuharu Kotani  Takeshi Kobayashi
Institution:(1) Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464 Nagoya, Japan;(2) Present address: Department of Chemical Engineering, Faculty of Engineering, Niigata University, Japan
Abstract:Summary High concentration cultivation of Bifidobacterium longum in a fermenter with cross-flow filtration using a ceramic filter is described. Continuous cross-flow filtration allowed complete recycling of the cells to the fermenter and also continuous separation of inhibitory metabolites. The final cell concentration attained in the cultivation was 54.4 g dry wt./l; this was seven times as high as that without cross-flow filtration. The time course of the cultivation with cross-flow filtration was predicted, based on the assumption that the specific growth rate can be expressed only as a function of concentrations of metabolites (acetate and lactate) in a culture broth.Nomenclature D dilution rate (h-1) - m maintenance coefficient (h-1) - OD 570 optimal density at 570 nm - P A acetate concentration (g/l) - P A0 initial acetate concentration (g/l) - P L lactate concentration (g/l) - P L0 initial lactate concentration (g/l) - S lactose (substrate) concentration (g/l) - S 0 initial lactose (substrate) concentration (g/l) - t cultivation time (h) - Y x/s growth yield (g/g) - X dry cell concentration (g/l) - X 0 initial dry cell concentration (g/l) - agr constant - beta constant
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