凝胶法生长溶菌酶晶体的研究

凝胶介质可以排除或削弱晶体生长过程中重力引起的对流和沉淀现象，用凝胶法生长生物大分子晶体是一种新的探索。使用类似于悬滴汽相扩散的方法，凝胶中生长出单个较大的外形发育完善且高度对称的鸡蛋清溶菌酶晶体。ＭＰＤ在凝胶中对溶菌酶结晶与溶液中具有相似的抑核作用。排循照像实验表明，凝胶法生长的晶体具有较强的衍射能力。

STUDIES ON THE GEl. TECHNIQUE FOR GROWING OF LYSOZYME CRYSTALS

The gel medium could eliminate or reduce the convection and sedimentation caused by the gravity and it is worthwhile to explore the gel method used for crystal growth of biological macromolecules. Using hanging drop diffustion method, we have grown single large or perfect and highly symmetrical crystals of hen - egg white lysozyme. In the gel medium. MPD in low concentration could inhibit the nucleation in lysozyme crystallization, which is similar to that in solution procedure. The X - ray precession photography showed that the crystals grown in gel could diffract to a higher resolution.