Protein deprivation attenuates Hsp expression in fat tissue |
| |
Authors: | Harel Eitam Rotem Agmon Aviv Asher Arieh Brosh Alla Orlov Ido Izhaki Ariel Shabtay |
| |
Institution: | (1) Institute of Animal Science, Department of Ruminant Science, Newe Ya’ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, 30095, Israel;(2) Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, 31905 Haifa, Israel; |
| |
Abstract: | For ruminants, dietary protein is the first limiting component to the utilization of low-quality forage. Throughout gestation,
low-protein intake may result in prenatal programming that causes various metabolic disturbances and physiological modulations
to dams and their developing embryos. We studied the effect of long-term low-protein diet (LPD) on physiological, biochemical,
and molecular parameters of the energy status in gestating beef cows. LPD resulted in significant reductions in feed intake
and heart rate and promoted a negative retained energy status already after 3 weeks. Elevated levels of plasma creatinine
and non-esterified fatty acids indicate endogenous degradation of fat and protein as a response to the demands in energy and
nitrogen. Increasing levels of β-hydroxybutyrate confirmed the negative energy status obtained by the physiological measurements.
At the molecular level, subcutaneous fat, Hsp90, Hsp70, and proteasome subunits decreased significantly after 3 months on
LPD, in parallel with an increase of adipocyte fatty acid-binding protein. These results may indicate a decrease in turn-over
of proteins, at the cost of induced lipolysis, and suggest that the response to protein deprivation, when examined in an energy-storing
tissue, includes downregulation of the constitutive heat shock proteins involved in the protein degradation pathway of energy
production and upregulation of tissue-specific genes such as those involved in energy production from fat degradation. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|