Optimization of bovine satellite cell-derived myotube formation in vitro |
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| Authors: | M V Dodson E L Martin M A Brannon B A Mathison D C McFarland |
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| Institution: | 1. Department of Marine Sciences and Convergent Technology, College of Science and Technology, Hanyang University, Ansan 426-791, Republic of Korea;2. Institute of Environmental and Energy Technology, POSTECH, Pohang 790-784, Republic of Korea;3. Marine Environment and Conservation Research Division, Korea Institute of Ocean Science and Technology (KIOST), Ansan 426-744, Republic of Korea;4. Marine Environment Research Division, National Fisheries Research and Development Institute (NFRDI), Busan 619-705, Republic of Korea;5. Wadsworth Center, New York State Department of Health, Albany, NY 12201-0509, USA;6. Department of Environmental Health Sciences, School of Public Health, State University of New York at Albany, Empire State Plaza, P.O. Box 509, Albany, NY 12201-0509, USA;7. Biochemistry Department, Faculty of Science and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, PO Box 80216, Jeddah 21589, Saudi Arabia;1. Water Science and Technology Directorate, Environment and Climate Change Canada, 867 Lakeshore Road, Burlington, ON L7S 1A1, Canada;2. Wildlife and Landscape Science Directorate, Environment and Climate Change Canada, 867 Lakeshore Road, Burlington, ON L7S 1A1, Canada;3. Department of Fisheries and Oceans, Maurice Lamontagne Institute, P.O. Box 1000, Mont-Joli, QC G5H 3Z4, Canada;2. The Institute of Medical Research, Israel-Canada, Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel |
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| Abstract: | Post-natal myogenic satellite cells, isolated from the sternomandibularis muscles of bovine at slaughter were used for primary culture studies. Isolated satellite cells tended to differentiate into multinucleated myotubes more efficiently if initially plated on to a fibronectin substratum. Bovine-derived satellite cells displayed greater fused cell numbers when exposed to Dulbecco's Modified Eagle's Medium (DMEM) supplemented with horse serum than similar supplementation with fetal calf serum (P less than 0.05) or sheep serum (P less than 0.05). In addition, differentiation appeared nearly complete after 4 days exposure to DMEM-1% horse serum as verified by beta-D-arabinofuranosyl-cytosine addition to cultures. Collectively, these data provide the first evidence that satellite cells can be isolated from a bovine skeletal muscle. Furthermore, these data indicate that bovine-derived satellite cells can be induced to undergo substantial morphological differentiation in vitro. |
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