THE ROLE OF PHOSPHOTASE AND ACETYLCHOLINESTERASE IN RESISTANCE TO PHOXIM IN HELICOVERPA ARMIGERA |
| |
Authors: | TANG Feng YUE Yong-de HUA Ri-mao |
| |
Institution: | Department of Plant Protection, Anhui Agricultural University, Hefei 230036, China |
| |
Abstract: | Abstract The resistance mechanism underlying phoxim resistance was investigated through biochemical assays. The results show that there are marked relations between the phosphatase activity and resistance to phoxim. Furthermore, there is a significant difference in the K i values of AChE between the two strains and it is inferred that the change in ability of binding to phoxim is a mechanism responsible for resistance to phoxim. The metabolic inhibitors Triphenyl Phosphate (TPP) and O,O-diethyl-O-phenyl-thiophosphate (SV1) displayed significant synergism of phoxim toxicity to the two strains. It implied that esterase might be involved in resistance. However, there is no marked correlation between resistance and CarE. |
| |
Keywords: | Helicoverpa armigera resistance mechanism acetylcholinesterase esterase phoxim |
|
|