Image analysis of restriction enzyme fingerprint autoradiograms |
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Authors: | Sulston, John Mallett, Frank Durbin, Richard Horsnell, Terry |
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Affiliation: | MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK |
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Abstract: | A genome mapping system has been developed that reads and assemblesdata from clones analysed by restriction enzyme fragmentationand polyacrylamide gel electrophoresis. Input data for the systemcan be most effectively obtained by the use of a scanning densitometerand image-processing package, such as that described in thisarticle. The image-processing procedure involves preliminarylocation of bands, cooperative tracking of lanes by correlationof adjacent bands, a precise densitometric pass, alignment ofthe marker bands with the standard, optional interactive editing,and normalization of the accepted bands. Received on August 31, 1988; accepted on December 6, 1988 |
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