Monitoring anthracycline binding to nucleotides by two dimensional cosy NMR experiments. |
| |
Authors: | B C Hammer R A Russell R N Warrener J G Collins |
| |
Institution: | Department of Chemistry, University College, University of New South Wales, Australian Defence Force Academy, Canberra. |
| |
Abstract: | The nucleotide binding ability of the novel anthracycline drug, 3-fluoro-4-demethoxydaunomycin, has been studied by two dimensional 1H NMR correlated spectroscopy (COSY). In the COSY spectrum of the nucleotide mini-helix d(CTGCAG)2 cross-peaks are observed from the spin-coupled H6 and H5 protons of the cytidine bases. Additional cytidine H6/H5 cross-peaks are observed in the COSY spectrum of the anthracycline-d(CTGCAG)2 complex. These additional cytidine cross-peaks enable the identification of the anthracycline binding sites and the determination of the relative kinetic stability of the bound drug at each binding site. |
| |
Keywords: | |
|
|