High frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus |
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| Authors: | Cristine Chaves Barreto,Luciano Cardoso Alves,Francisco José Lima Aragã o,El&# &# bio Rech,Augusto Schrank,Marilene Henning Vainstein |
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| Affiliation: | Departamento de Biologia Celular, Universidade de Brasıćlia, 70910-900 Brasıćlia, DF, Brazil;Cenargen/Embrapa, 70849-970 Brasıćlia, DF, Brazil;Centro de Biotecnologia, Departamento de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, 90540-000 Porto Alegre, RS, Brazil |
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| Abstract: | Two different methods, (i) PEG and (ii) biolistic, were employed to transform protoplasts and conidia of Paecilomyces fumosoroseus using hygromycin resistance as selectable marker. Transformation frequencies varied from 1.9 to 2.5 transformants μg−1 of DNA by the PEG method, and from 33 to 153 transformants μg−1 of DNA by the biolistic procedure. |
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| Keywords: | Paecilomyces fumosoroseus Fungal transformation Biolistic Hygromycin B resistance Microprojectile bombardment |
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