Lectin histochemistry of normal human gastric mucosa |
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Authors: | Chong Jiang Sheena F McClure Robert W Stoddart John McClure |
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Institution: | (1) Laboratory Medicine Academic Group, School of Medicine, University of Manchester, Oxford Road, Manchester, M13 9PT |
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Abstract: | Information about the saccharides expressed in gastric mucosa is mostly limited to the glycan content of gastric mucins and
there are only a few studies of the glycoprofiling of the constituent cells and their components. Knowledge of the glycan
expression of normal gastric mucosa is necessary for the interpretation of the significance of changes of expression in disease.
A lectin histochemical study of normal human gastric (body) mucosa was performed using 27 lectins chosen to probe for a wide
range of oligosaccharide sequences within several categories of glycoprotein glycans.
There were marked differences in staining reactions in the various microanatomical structures of the mucosa, particularly
between pits and glands with the former more closely resembling the surface epithelium. A notable feature was the degree of
difference in the staining between a substantial sub-population of cells within the neck region and the epithelium of both
the pits and glands. These neck cells resembled the pit cells with some lectins, glandular cells with some others and neither
with some other lectins. Overall, the differences between the pit, gland and neck epithelia were diverse and numerous, and
could not be explained by altered activity of a small set of glycosyltransferases. Widespread alterations of glycans must
have occurred (affecting terminal and internal parts of their structures) and the very different glycotypes of the pit, neck
and gland epithelia are, therefore, suggestive of the existence of three cell lineages within normal gastric epithelium. Published in 2004.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | gastric mucosa lectin glycan |
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