高尔基体在人胃腺癌细胞诱导分化过程中的变化

MGc 80-3细胞高尔基体呈发育差、结构不典型状态,但经dBcAMP诱导后,细胞内高尔基体组数增多、分布集中、体积增大,高尔基囊数目增多、排列规则,囊的膜内颗粒增多、分布较为均匀,恢复为与其相应正常细胞相似、发育良好的典型高尔基体结构。这种变化不仅抑制了胃癌细胞的恶性分泌活动,同时对细胞表面成份的变化也起着一定的调节作用。认为高尔基体结构与功能向典型方向的转??变是癌细胞恶性表型逆转的一种重要表现,对于癌细胞由恶性向正常方向的分化具有重要影响。

CHANGES OF GOLGI COMPLEX DURING THE INDUCED DIFFERENTIATION OF HUMAN GASTRIC ADENOCARCINOMA CELLS IN VITRO

Golgi complex is a central link relatedto each kind of organella in cellular meta-bolic process,its morphological charges areoften concerned with the cell differentiationand functional state.The Golgi complexes intumour cells have the characteristics of poordevelopment and non-typical structure,andclosely related to their pathological differen-tial degree.For this reason,having based onappraising the differential effect of humangastric adenocarcinoma cell line MGc 80-3induced by dBcAMP in vitro,we had madea systematic observation on the changes ofGolgi complex during the malignant pheno-typical reversion of MGc 80-3 cells,in orderto inquire into the relationship between thestructural and functional changes of Golgicomplex and the malignant phenotypic re-version of cancer cells. It was revealed by ultrathin sectioningand freeze-etching electron microscopy that,in MGc 80-3 cells,there were a few Golgicomplexes,their volume was small,the am-ounts of saccule were few,they arrangedirregularly,expanded and inflated,and theintramembranous particles on saccule wererare and not well-distributed.It displayeda non-developmental and non-typical struc-tural state of Golgi complex.But after in-duced treatment with dBcAMP,the Golgicomplexes had grown in number and distri-buted concentratedly,their volume enlarged,the saccules increased and arranged regularly,and the intermembranous particles on saccu-les were plentiful and well-distributed.Theyhad restored a well-developed and typicalstructure of Golgi complex similar to thatof the primary culture cell of human normalgastric mucous membrane. It showed that Golgi complex had fur-ther changed into a quite developed stateduring the induced differentiation.This al-teration not only inhibited the malignantsecretory activity of gastric carcinoma cellsbut also played a certain regulative role inthe changes of the surface components ofcancer cells.The structural and functionalchanges of Golgi complex were considered tobe an important expression in the malignantphenotypical reversion of cancer cells,it hadan important influence on the differentiationof cancer cells from malignant to normaldirection.