|
|||||
|
|
Efficient Somatic Embryogenesis in Alstroemeria |
|
| Authors: | J B Kim C J J M Raemakers E Jacobsen R G F Visser |
| Institution: | (1) Laboratory of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands;(2) Central Research Institute, Neobio Co., Ltd, #26-1 Sadong-ri, Dae-Weol Myeon, Icheon, Kyong-ki, 467-854, Korea |
| Abstract: | In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 μM thidiazuron and 0.5 μM indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 μM 2,4-dichlorophenoxy acetic acid and 2.2 μM benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 μM picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 μM BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally. |
| Keywords: | Alstroemeria Compact embryogenic callus Friable embryogenic callus Regeneration Somatic embryos |
| 本文献已被 SpringerLink 等数据库收录！ | |